Journal: Journal of Biomedical Optics

Article Title: Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging

doi: 10.1117/1.JBO.17.10.106002

Figure Lengend Snippet: Cross-Polarization OCT. Housed in the probe casing was a Mach-Zehnder type interferometer that used a polarization beam splitter (PBS) to illuminate a two-axis tilt MEMS scanning mirror with linearly polarized light (P). Light from a swept source 1310-nm ...

Article Snippet: Cross-Polarization Optical Coherence Tomography A customized cross-polarization swept source OCT (CP-OCT) system with an intraoral probe (Fig. ) was used (IVS-200-CPM, Santec Co. Komaki, Japan) for in vitro dental materials imaging.

Techniques:

Journal: Biomedical Optics Express

Article Title: Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

doi: 10.1364/BOE.533339

Figure Lengend Snippet: Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining D-OCT viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto custom 3D-printed plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.

Article Snippet: The horizontal, vertical, and in-plane pixel dimensions were sampled at 256 × 256 × 256 px , resulting in a lateral pixel separation of 12.18 μ m and an axial separation was 6.996 μ m . Custom OCT imaging plates were 3D printed (Formlabs), with 3 × 3 × 0.5 mm well for spheroid imaging.

Techniques: Biomarker Discovery, Imaging, Trypan Blue Exclusion Assay, Produced

Journal: Biomedical Optics Express

Article Title: Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

doi: 10.1364/BOE.533339

Figure Lengend Snippet: Normalisation techniques to decouple motion from intensity levels. a. Sample spheroids demonstrated results of 3 variance formulations on motion estimation. Relative deviation (Eq. ) exhibits similar patterns to intensity variation (Eq. ). Our regression-adjusted variance approach removed bias from low intensity near the bottom of spheroids. OCT images shown are illustrative examples and do not reflect the data distribution, which is better presented in the box plot. Colour bars were adjusted for optimal visual clarity. b. Median motion (Eq. ) distributions of 60 spheroids per viability insult group. MIV exhibits a large range, unable to discern between spheroid groups. Regression adjustment reduces spread and improves separation. Viability distribution obtained via trypan blue exclusion shows high correspondence with MAV distributions.

Article Snippet: The horizontal, vertical, and in-plane pixel dimensions were sampled at 256 × 256 × 256 px , resulting in a lateral pixel separation of 12.18 μ m and an axial separation was 6.996 μ m . Custom OCT imaging plates were 3D printed (Formlabs), with 3 × 3 × 0.5 mm well for spheroid imaging.

Techniques: